Stabilization of rifamycin-B oxidase fromCurvularia lunata
โ Scribed by S. S. Yadav; Geeta Kanjilal; D. V. Singh
- Book ID
- 105244137
- Publisher
- Springer-Verlag
- Year
- 1992
- Tongue
- English
- Weight
- 383 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1476-5535
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๐ SIMILAR VOLUMES
Rifarnycin oxidase of C~rvulrm'a l~~tatu was immobilized on polyacrylamide \_eel. The optimum pH and temperature for immobilized enzyme reaction were 6.5 and 50ยฐC, respectively. Enzyme stability increased on immobilization and the half lives of immobilized enzyme preparations at 30 and 40ยฐC were 30
Maximum activity (8.9 IU/ml) of rifamycin oxidase in Curuuhia Zunata, grown in shake-flask culture at 28ยฐC and pH 6.5, was after 96 h. Nearly all the glucose was used in 72 h. An initial culture pH of 6.5 and 28ยฐC were optimum for the growth and enzyme production. Among various carbon and organic ni