Optimization of culture conditions for the production of rifamycin oxidase byCurvularia lunata

✍ Scribed by U.C. Banerjee

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 246 KB
• Volume: 10
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00144473

✦ Synopsis

Maximum activity (8.9 IU/ml) of rifamycin oxidase in Curuuhia Zunata, grown in shake-flask culture at 28°C and pH 6.5, was after 96 h. Nearly all the glucose was used in 72 h. An initial culture pH of 6.5 and 28°C were optimum for the growth and enzyme production. Among various carbon and organic nitrogen sources, carboxymethylcellulose and peptone were the most effective for enzyme yield. The rate of enzyme production was enhanced when yeast extract was also added to the medium. The optimum medium for the production of rifamycin oxidase contained 10 g each of yeast extract, peptone and carboxymethylcellulose/l and 0.04 % (NH,l,SO,.