Transformation of rifamycin B with immobilized rifamycin oxidase ofCurvularia lunata

✍ Scribed by Uttam Chand Banerjee

• Book ID: 104644664
• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 352 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00152538

✦ Synopsis

Rifarnycin oxidase of C~rvulrm'a l~~tatu was immobilized on polyacrylamide _eel. The optimum pH and temperature for immobilized enzyme reaction were 6.5 and 50°C, respectively. Enzyme stability increased on immobilization and the half lives of immobilized enzyme preparations at 30 and 40°C were 30 and 11.5 d, respectively. With 2.5 mm beads diffusional resistances were observed. Reusability studies showed that 1 mm size beads gave a higher rate of transformation in comparison with 2 or ?._S mm beads.