Stability enhancement of Escherichia coli penicillin G acylase by glycosylation with yeast mannan
✍ Scribed by Jana Masárová; Danica Mislovičová; Peter Gemeiner; Eva Michalková
- Book ID
- 111138489
- Publisher
- Portland Press
- Year
- 2001
- Tongue
- English
- Weight
- 127 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0885-4513
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📜 SIMILAR VOLUMES
The stabilisation of Escherichia coli penicillin G acylase (PGA) by dextran polymers (of molecular weight 11.5, 37.7 and 71 kDa) was studied. The inactivation of both the native and dextrancontaining enzyme preparations obeyed ®rst-order kinetics at the temperature and pH values studied. The optimal
Different techniques including toluene-ethanol, guanidine hydrochloride, guanidine hydrochloride-EDTA, lysozyme, lysozyme-EDTA and sonication treatments were used to extract penicillin G acylase from Escherichia coli ATCC 11105 cells. The obtained results show that penicillin G acylase extraction by