Video microscopy has been used to investigate the conformational changes that double stranded DNA undergoes during capillary electrophoresis in buffered solutions of hydroxyethyl cellulose (HEC). DNA of a fixed size (T2, 167 kbp) was used to quantitate the degree of extension. At HEC concentrations
Spectroscopic Study of Orientation Dynamics of DNA during Electrophoresis in Entangled and Dilute Polyacrylamide Solutions
โ Scribed by Carlsson, Christina; Jonsson, Mats
- Book ID
- 126041138
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 307 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0024-9297
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## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m
Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g