Dynamics of single-stranded DNA in polyacrylamide gels during pulsed field gel electrophoresis. A birefringence study
β Scribed by Jean Sturm; Alain Pluen; Gilbert Weill
- Book ID
- 107944273
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 389 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0301-4622
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π SIMILAR VOLUMES
## Abstract Using a computer simulation algorithm based on the reptation model, we investigated the effects of pulsed field gel electrophoresis on the separation of singleβstranded DNA molecules in denaturing polyacrylamide gels. Pulsed fields that combine two different field intensities were found
## Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis We describe an original apparatus for the study of the dynamics of single stranded DNA migration. Four detectors based on laser-induced fluorescence (LIF) are equidistantly placed on one migration la
## Dynamics of DNA under pulsed-field gel electrophoresis as analyzed from birefringence rise and decay The orientation of DNA induced by electrophoretic transport in agarose gel has been studied by optical birefringence. From its field-free decay, it is clearly demonstrated that the degree of ori