Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis

✍ Scribed by Zahia Djouadi; Samuele Bottani; Marie-Alix Duval; Rainer Siebert; Hrvé Tricoire; Luc Valentin

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 161 KB
• Volume: 22
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(200109)22:16<3527::aid-elps3527>3.0.co;2-6

✦ Synopsis

Dynamics of single-stranded DNA migration in denaturing polyacrylamide slab-gel electrophoresis

We describe an original apparatus for the study of the dynamics of single stranded DNA migration. Four detectors based on laser-induced fluorescence (LIF) are equidistantly placed on one migration lane, allowing repeated measurements of the same DNA band at different positions along migration. This article presents the characteristics and performances of this system and focuses on the data analysis, showing how the multiple detection scheme enables the study of band broadening and band resolution during a migration run. Our results suggest the existence of anomalous (nonthermal) diffusion of DNA molecules during the electrophoretic process.