Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g
Capillary electrophoresis of DNA fragments in entangled polymer solutions: A study of separation variables
✍ Scribed by Vladislav Dolník; Milos Novotny
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 351 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled matrix for the separation of DNA fragments has been demonstrated.
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