๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Dynamics of T2 DNA during electrophoresis in entangled and ultradilute hydroxyethyl cellulose solutions

โœ Scribed by Richard W. Hammond; Xuelong Shi; Michael D. Morris

Book ID
102660742
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
825 KB
Volume
8
Category
Article
ISSN
1040-7685

No coin nor oath required. For personal study only.

โœฆ Synopsis

Video microscopy has been used to investigate the conformational changes that double stranded DNA undergoes during capillary electrophoresis in buffered solutions of hydroxyethyl cellulose (HEC). DNA of a fixed size (T2, 167 kbp) was used to quantitate the degree of extension. At HEC concentrations below the entanglement limit, C*, extension of DNA under DC fields is much less than in gels. Pulsed fields serve to limit extension even further, with DNA strands approaching a time-averaged irregular conformation that yields rapid sizing of long nucleic acids.

๐Ÿ“œ SIMILAR VOLUMES

Experimental and theoretical studies of
Experimental and theoretical studies of DNA separations by capillary electrophoresis in entangled polymer solutions
โœ Paul D. Grossman; David S. Soane ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 681 KB

Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g

Capillary electrophoresis of DNA fragmen
Capillary electrophoresis of DNA fragments in entangled polymer solutions: A study of separation variables
โœ Vladislav Dolnรญk; Milos Novotny ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 351 KB

## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m