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Capillary Electrophoresis of Supercoiled and Linear DNA in Dilute Hydroxyethyl Cellulose Solution

โœ Scribed by Hammond, Richard W.; Oana, Hidehiro; Schwinefus, Jeffrey J.; Bonadio, Jeffrey; Levy, Robert J.; Morris, Michael D.

Book ID
126855470
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
286 KB
Volume
69
Category
Article
ISSN
0003-2700

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Dynamics of T2 DNA during electrophoresi
Dynamics of T2 DNA during electrophoresis in entangled and ultradilute hydroxyethyl cellulose solutions
โœ Richard W. Hammond; Xuelong Shi; Michael D. Morris ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 825 KB

Video microscopy has been used to investigate the conformational changes that double stranded DNA undergoes during capillary electrophoresis in buffered solutions of hydroxyethyl cellulose (HEC). DNA of a fixed size (T2, 167 kbp) was used to quantitate the degree of extension. At HEC concentrations