Inactivation of the protease inhibitor p
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Gordon T. James
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Article
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1978
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Elsevier Science
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English
โ 315 KB
Aqueous preparations ofphenylmethylsulfonyl fluoride (PMSF) become inactive toward proteases unless promptly brought into contact with protease. Inactivation of PMSF increases with increased pH and temperature. Half-lives ofthe inhibitor at 25ยฐC are approximately I10,55, and 35 min at pH 7.0,7.5, an