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Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers

✍ Scribed by Gordon T. James

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 315 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90784-4

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✦ Synopsis

Aqueous preparations ofphenylmethylsulfonyl fluoride (PMSF) become inactive toward proteases unless promptly brought into contact with protease. Inactivation of PMSF increases with increased pH and temperature. Half-lives ofthe inhibitor at 25°C are approximately I10,55, and 35 min at pH 7.0,7.5, and 8.0. respectively. At pH 8, 100 /.LM PMSF

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