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SORS: A Universal One-Round PCR-Based Method for Site-Directed Mutagenesis

✍ Scribed by Bao-Li Wang; Jin-Xing Quan; Hui Liang; Yan-Li Jiao; Xiao-Xia Li; Gang Guo; Jing-Yu Zhang

Book ID
107581613
Publisher
Humana Press Inc
Year
2008
Tongue
English
Weight
338 KB
Volume
40
Category
Article
ISSN
1073-6085

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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir