Multiple Site Directed Mutagenesis Strategy Based on Total RNA and RT-PCR Method
✍ Scribed by Zsolt B. Nagy; Márk Gárdonyi; Attila Mészáros; Zoltán Varga-Orvos; Robert G. Solomon; László Tamás
- Book ID
- 107581519
- Publisher
- Humana Press Inc
- Year
- 2007
- Tongue
- English
- Weight
- 296 KB
- Volume
- 37
- Category
- Article
- ISSN
- 1073-6085
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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir