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A novel simple and rapid PCR-based site-directed mutagenesis method

✍ Scribed by Imen Rabhi; Naouel Guedel; Imen Chouk; Khaled Zerria; M. Ridha Barbouche; Koussay Dellagi; Dahmani M. Fathallah

Book ID
111640371
Publisher
Humana Press Inc
Year
2004
Tongue
English
Weight
141 KB
Volume
26
Category
Article
ISSN
1073-6085

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πŸ“œ SIMILAR VOLUMES

A rapid and highly efficient method for
A rapid and highly efficient method for PCR-based site-directed mutagenesis using only one new primer
✍ Eckhard Boles; Thomas Miosga πŸ“‚ Article πŸ“… 1995 πŸ› Springer-Verlag 🌐 English βš– 170 KB

We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir