185 Rapid and efficient site-directed mutagenesis by single-tube ‘megaprimer’ PCR method
✍ Scribed by Ke, Song Hua (author);Madison, Edwin L. (author)
- Book ID
- 117439562
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 126 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0268-9499
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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir