A rapid and highly efficient method for
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Eckhard Boles; Thomas Miosga
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Article
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1995
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Springer-Verlag
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English
โ 170 KB
We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir