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A one-step PCR-based method for rapid and efficient site-directed fragment deletion, insertion, and substitution mutagenesis

โœ Scribed by Dong Qi; Karen-Beth G. Scholthof


Book ID
113767746
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
234 KB
Volume
149
Category
Article
ISSN
0166-0934

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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir