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A rapid and efficient method for multiple-site mutagenesis with a modified overlap extension PCR

โœ Scribed by Yingfeng An; Jianfei Ji; Wenfang Wu; Anguo Lv; Ribo Huang; Yutuo Wei


Publisher
Springer
Year
2005
Tongue
English
Weight
144 KB
Volume
68
Category
Article
ISSN
1432-0614

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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir