Production of fertile somatic hybrid pla
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Sergei Krasnyanski; Laszlo Menczel
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Article
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1995
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Springer
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English
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Protoplasts of Helianthus giganteus and Helianthus annuus were fused using polyethylene glycol. Before fusion H.giganteus protoplasts were subjected to iodoacetic acid treatment to render them unable to divide. Fused protoplasts were cultured in V-KM medium containing benzylaminopurine and naphtalen