Somatic hybrid plants obtained by protoplast fusion betweenCitrus sinensisandPoncirus trifoliata
โ Scribed by T. Ohgawara; S. Kobayashi; E. Ohgawara; H. Uchimiya; S. Ishii
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 930 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0040-5752
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Protoplasts of Helianthus giganteus and Helianthus annuus were fused using polyethylene glycol. Before fusion H.giganteus protoplasts were subjected to iodoacetic acid treatment to render them unable to divide. Fused protoplasts were cultured in V-KM medium containing benzylaminopurine and naphtalen
PEG4nduced fusion of two haploid Saccharomyces strains resulted in three morphologically different types of fusion products. In order to estimate the respective ploidy, one representative of each type -F/, F2 and F3 -was subjected to a measurement of the cellular DNA content and to a meiotic segrega