Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an
Regeneration of intergeneric somatic hybrids by protoplast fusion betweenOnobrychis viciaefoliaandMedicago sativa
โ Scribed by Ziqin Xu; Jingfen Jia
- Publisher
- Science in China Press (SCP)
- Year
- 1997
- Tongue
- English
- Weight
- 508 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1674-7305
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Protoplasts of Helianthus giganteus and Helianthus annuus were fused using polyethylene glycol. Before fusion H.giganteus protoplasts were subjected to iodoacetic acid treatment to render them unable to divide. Fused protoplasts were cultured in V-KM medium containing benzylaminopurine and naphtalen