Production of interspecific somatic hybrid plants in the genusMedicagothrough protoplast fusion
β Scribed by E Pupilli; G. M. Scarpa; F. Damiani; S. Arcioni
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 676 KB
- Volume
- 84-84
- Category
- Article
- ISSN
- 0040-5752
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Protoplasts of Helianthus giganteus and Helianthus annuus were fused using polyethylene glycol. Before fusion H.giganteus protoplasts were subjected to iodoacetic acid treatment to render them unable to divide. Fused protoplasts were cultured in V-KM medium containing benzylaminopurine and naphtalen
1. No hybrid plants of Nicotiana tabacum + Petunia hybrida were regenerated from calluses of fusion experiments with mesophyll protoplasts of N. tabacum s, s (2) and v and of P. hybrida mu 1 (2). 2. After in vitro pollination of ovules of N. tabacum with pollen of P. hybrida, filamentous proembryos
An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size g