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Solution properties of synthetic polypeptides. XVIII: Helix–coil transition of poly-N5-(2-hydroxyethyl)L-glutamine

✍ Scribed by Michihisa Miyake; Shuichi Akita; Akio Teramoto; Takashi Norisuye; Hiroshi Fumita

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
568 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The helix–coil transition of poly‐N^5^‐(2‐hydroxyethyl)L‐glutamine (PHEG) in aqueous isopropanol was examined by means of optical rotatory dispersion (ORD) and intrinsic viscosity [η] measurements. The Zimm–Bragg parameters σ and s for the transition were determined from the ORD data as a function of molecular weight. It was found that the transition was characterized by a relatively low cooperativity; the values of \documentclass{article}\pagestyle{empty}\begin{document}$ \sqrt \sigma $\end{document} were in the range from 0.039 to 0.066, depending on the solvent composition. These σ values are much larger than those reported for other polypeptide–solvent systems. The transition enthalpy was negative and its magnitude varied with the solvent composition, with a maximum of 620 cal/mol at 40 wt% isopropanol. The curve of [η] versus helical content for a high‐molecular‐weight sample exhibited a very broad minimum, and this behavior was attributed to the low cooperativity of the transition.

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