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Solubilization and partial characterization of particulate dehydrogenases from Clostridium kluyveri

✍ Scribed by Peter Hillmer; Gerhard Gottschalk


Book ID
115733591
Publisher
Elsevier Science
Year
1974
Weight
719 KB
Volume
334
Category
Article
ISSN
0005-2744

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Simultaneous single-step purification of
✍ Mark X. Sliwkowski; Maris G.N. Hartmanis πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 795 KB

Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1