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Purification and characterization of a coenzyme-A-dependent succinate-semialdehyde dehydrogenase from Clostridium kluyveri

✍ Scribed by Brigitte SÖHLING; Gerhard GOTTSCHALK


Book ID
115129866
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
882 KB
Volume
212
Category
Article
ISSN
1432-1327

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✍ Mark X. Sliwkowski; Maris G.N. Hartmanis 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 795 KB

Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1