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Purification and Characterization of the Oxygen-Sensitive 4-Hydroxybutanoate Dehydrogenase from Clostridium kluyveri

✍ Scribed by R.A. Wolff; W.R. Kenealy

Book ID
115644909
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
667 KB
Volume
6
Category
Article
ISSN
1046-5928

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Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1