Simultaneous single-step purification of
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Mark X. Sliwkowski; Maris G.N. Hartmanis
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Article
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1984
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Elsevier Science
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English
β 795 KB
Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1