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Purification and Properties of NADP-Dependent l(+)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridium kluyveri

✍ Scribed by Vijay K. Madan; Peter HILLMER; Gerhard Gottschalk


Book ID
115113434
Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
640 KB
Volume
32
Category
Article
ISSN
1432-1327

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Simultaneous single-step purification of
✍ Mark X. Sliwkowski; Maris G.N. Hartmanis πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 795 KB

Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1