Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy
✍ Scribed by Donghua H. Zhou, Andrew J. Nieuwkoop, Deborah A. Berthold, Gemma Comellas, Lindsay J. Sperling, Ming Tang, Gautam J. Shah, Elliott J. Brea, Luisel R. Lemkau, Chad M. Rienstra
- Book ID
- 118741344
- Publisher
- Springer Netherlands
- Year
- 2012
- Tongue
- English
- Weight
- 873 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0925-2738
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## Abstract **The natural way**: A sensitive NMR spectroscopic method is developed to obtain well‐resolved two‐dimensional spectra (^15^N–^1^H and ^13^C–^1^H) for natural‐abundance (that is, without the need for isotopic enrichment) large‐molecule samples, such as biopharmaceuticals. This method gi
It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable