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Solid Phase Synthesis of Peptide C-Terminal Thioesters by Fmoc/ t -Bu Chemistry

โœ Scribed by Ingenito, Raffaele; Bianchi, Elisabetta; Fattori, Daniela; Pessi, Antonello


Book ID
120073728
Publisher
American Chemical Society
Year
1999
Tongue
English
Weight
81 KB
Volume
121
Category
Article
ISSN
0002-7863

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To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact.