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An improved deblocking agent for direct Fmoc solid-phase synthesis of peptide thioesters

✍ Scribed by Xianzhang Bu; Guiyang Xie; Chi Wang Law; Zhihong Guo

Publisher: Elsevier Science
Year: 2002
Tongue: French
Weight: 133 KB
Volume: 43
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(02)00237-x

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✦ Synopsis

To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact. The deprotecting reagent, consisting of DBU and HOBt, was successfully used to prepare a decapeptide in high yield.

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