Direct Fmoc/tert-Bu solid phase synthesis of octamannosyl polylysine dendrimer–peptide conjugates
✍ Scribed by Eric Assen B. Kantchev; Chung-Chieh Chang; Ding-Kwo Chang
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2006
- Tongue
- English
- Weight
- 322 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
The mannose binding proteins on the surface of the dendritic cells are responsible for capture of pathogens in the early stages of immune response. Conjugation to mannose dendrimers is a rarely explored but potentially powerful strategy for enhancing immunogenicity of synthetic peptides relying on direct delivery to dendritic cells. We describe a general protocol for preparation of pure, monodisperse third‐generation mannosylated poly‐L‐lysine dendrimer‐peptide conjugates using direct, machine‐assisted Fmoc/t‐Bu solid phase peptide synthesis. The glycodendrons were elaborated onto the N‐ or C‐terminus of sequences derived from HIV‐1 gp41, SARS‐CoV S2 protein, and Influenza Hemagglutinin (consisting of 15–44 residues). The products were obtained in a homogeneous state after cleavage from the resin, deprotection, and a single purification on semipreparative RP–HPLC. © 2005 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 84: 232–240, 2006
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