A straightforward stepwise method for the preparation of peptide-oligonucleotide phosphorothioate conjugates, was developed, based on the highly efficient Fmoc peptide solid phase synthesis, followed by oligonucleotide phosphorothiate chain assembly. The three conjugates synthesized contained 15-or
Solid phase synthesis of a cyclic peptide using fmoc chemistry
β Scribed by John S. McMurray
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- French
- Weight
- 282 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
Aiming at the direct preparation of peptide thioesters by an Fmoc solid-phase method, we searched a new deblocking reagent, which efficiently removed Fmoc groups while keeping the thioester intact. The deblocking reagent, which contains 1-methylpyrrolidine, hexamethyleneimine and HOBt in a one to on
Established methodology for the preparation of peptide thioesters requires the use of t-butoxycarbonyl chemistry owing to the lability of thioester linkers to the nucleophilic reagents used in Fmoc solid phase peptide synthesis. Both the greater ease of use and the broad applicability of the method