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Solid-phase Synthesis and Cellular Localization of a C- and/or N-terminal Labelled Peptide

✍ Scribed by P. Vidal; L. Chaloin; J. Méry; N. Lamb; N. Lautredou; R. Bennes; F. Heitz

Book ID
105360392
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
672 KB
Volume
2
Category
Article
ISSN
1075-2617

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✦ Synopsis

We report the solid-phase synthesis by the Fmoc strategy of a peptide containing a cysteamide group at its C-terminus. This peptide was subjected to further modifications including the linkage of fluorophores, namely lucifer yellow and coumarin respectively, at the C- and/or N-terminals. After incubation with living cultured cells these two probes were localized and it is concluded that the post-synthesis modifications can strongly modify the localization of the peptide.

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