Sodium dodecyl sulfate agarose gel electrophoresis: Stacking of the samples in a short polyacrylamide layer

✍ Scribed by Abdelkébir Rhalem; Dr. Pierre Pery

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 427 KB
• Volume: 6
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150061110

✦ Synopsis

A method is described for obtaining a sample-stacking effect before sodium dodecyl sulfate-agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide-agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be separated, according to the molecular weight of their subunits, in 15 % w/w agarose gels. The new gel system is versatile and may offer advantages over sodium dodecyl sulfate -polyacrylamide gel electrophoresis in such applications as protein elution or enzyme renaturation.