Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using colloidal silver
✍ Scribed by Dr. Pavel Dráber
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 386 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0173-0835
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📜 SIMILAR VOLUMES
A method is described for obtaining a sample-stacking effect before sodium dodecyl sulfate-agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide-agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be s
Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip