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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis without a stacking gel: Use for high sample throughput

✍ Scribed by Dr. Thomas Marshall; S. J. Stephen Gaston; Katherine M. Williams


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
382 KB
Volume
10
Category
Article
ISSN
0173-0835

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A method is described for obtaining a sample-stacking effect before sodium dodecyl sulfate-agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide-agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be s