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Single-Molecule Imaging of RNA Polymerase-DNA Interactions in Real Time

โœ Scribed by Harada, Yoshie; Funatsu, Takashi; Murakami, Katsuhiko; Nonoyama, Yoshikazu; Ishihama, Akira; Yanagida, Toshio

Book ID
119412222
Publisher
Biophysical Society
Year
1999
Tongue
English
Weight
266 KB
Volume
76
Category
Article
ISSN
0006-3495

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## Abstract **One's enough**: The direct observation of a DNAโ€polymeraseโ€based โ€œsequencing engineโ€ using singleโ€molecule detection recently allowed singleโ€molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5โ€ฒโ€phosphate unit and zeroโ€mode waveguides are cruci