Sequencing Single DNA Molecules in Real Time

✍ Scribed by Jenny Ibach; Susanne Brakmann

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 652 KB
• Volume: 48
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.200900722

✦ Synopsis

Abstract

One's enough: The direct observation of a DNA‐polymerase‐based “sequencing engine” using single‐molecule detection recently allowed single‐molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5′‐phosphate unit and zero‐mode waveguides are crucial components of this approach, which at last promises low‐cost genome‐scale sequencing.magnified image