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Real-Time DNA Sequencing from Single Polymerase Molecules

โœ Scribed by Eid, J.; Fehr, A.; Gray, J.; Luong, K.; Lyle, J.; Otto, G.; Peluso, P.; Rank, D.; Baybayan, P.; Bettman, B.

Book ID
111645139
Publisher
American Association for the Advancement of Science
Year
2009
Tongue
English
Weight
923 KB
Volume
323
Category
Article
ISSN
0036-8075

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## Abstract **One's enough**: The direct observation of a DNAโ€polymeraseโ€based โ€œsequencing engineโ€ using singleโ€molecule detection recently allowed singleโ€molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5โ€ฒโ€phosphate unit and zeroโ€mode waveguides are cruci