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Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes

✍ Scribed by Götz Kohler; Florian Rost; Joachim Seelig

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
144 KB
Volume
58
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Intracellular/extracellular lactate (Lac) distribution has been determined before in human and animal erythrocytes (red blood cells [RBCs]) with various methods. However, all previous methods determine intra‐ and extracellular Lac separately or indirectly. Now, ^13^C‐NMR spectroscopy has been used to monitor intra‐ and extracellular Lac simultaneously in intact RBCs. Isolated human RBCs were incubated with [3‐^13^C]‐Lac, [3‐^13^C]‐pyruvate (Pyr), and [1‐^13^C]‐glucose (Gluc). A distortionless enhancement by polarization transfer (DEPT) sequence was used (TR = 3.3 s, N = 128) to monitor the ^13^C‐NMR resonances in both compartments. The intra‐ and extracellular methyl group resonances of Lac and Pyr were clearly separated by 9.6 Hz and 7.0 Hz, respectively, under normoxic conditions due to the RBC chemical‐shift effect. The results show that the chemical‐shift effect of RBCs is convenient to monitor intra‐ and extracellular Lac simultaneously in intact RBCs under normoxic conditions. Magn Reson Med 58:213–217, 2007. © 2007 Wiley‐Liss, Inc.

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