Direct observation of resolved intracellular and extracellular water signals in intact human red blood cells using 1H MAS NMR spectroscopy
✍ Scribed by Eberhard Humpfer; Manfred Spraul; Andrew W. Nicholls; Jeremy K. Nicholson; John C. Lindon
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 266 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
High resolution 400 MHz ^1^H NMR spectra of red blood cell suspensions when measured using magic angle spinning (MAS) show two water resonances separated by 15 Hz. Based on addition of a paramagnetic Mn‐EDTA complex, measurement of relaxation times and variation of extracellular H~2~O/D~2~O ratios, these have been assigned as intracellular (line‐width 17.5 Hz) and extracellular water (linewidth 4.6 Hz). This is the first direct observation of intracellular water using NMR spectroscopy and the ^1^H MAS NMR spectroscopic approach offers the possibility of studying directly the compartmentation of substances in cells and kinetics of molecular transport.