𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation of intra- and extracellular lactate NMR signals using a lanthanide shift reagent

✍ Scribed by Silvio Aime; Mauro Botta; Valentina Mainero; Enzo Terreno

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
83 KB
Volume
47
Category
Article
ISSN
0740-3194

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

This work describes the use of [Pr‐DO3A] as a shift reagent for differentiating intra‐ and extracellular L‐lactate resonance in ^1^H‐ and ^13^C‐NMR spectra. DO3A acts as heptadentate ligand towards lanthanide(III) ions, leaving two coordination vacancies for the coordination of the L‐lactate ion. The exchange between free and [Pr‐DO3A]‐bound L‐lactate is fast on the NMR timescale, thus yielding a paramagnetically shifted L‐lactate signal for the substrate in the compartment containing the paramagnetic chelate. The evaluation of the method was carried out on a model system based on sealed ghosts from human red blood cells. Magn Reson Med 47:10–13, 2002. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Simultaneous separation of intracellular
Simultaneous separation of intracellular and extracellular lactate NMR signals of human erythrocytes
✍ Götz Kohler; Florian Rost; Joachim Seelig 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 144 KB

## Abstract Intracellular/extracellular lactate (Lac) distribution has been determined before in human and animal erythrocytes (red blood cells [RBCs]) with various methods. However, all previous methods determine intra‐ and extracellular Lac separately or indirectly. Now, ^13^C‐NMR spectroscopy ha

Enantiomeric differentiation of acyclic
Enantiomeric differentiation of acyclic terpenes by 13C NMR spectroscopy using a chiral lanthanide shift reagent
✍ Marie-Cécile Blanc; Pascale Bradesi; Joseph Casanova 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 93 KB

The 13C NMR behaviour of ten acyclic terpene alcohols was examined in the presence of a chiral lanthanide shift reagent (CLSR). For each alcohol, we measured the lanthanide-induced shift (LIS) on the signals of the carbons and the splitting of some signals, which allowed the enantiomeric differentia

1H NMR separation of epimeric mixtures b
1H NMR separation of epimeric mixtures by lanthanide shift reagents: Analysis of some isoxazolidine derivatives
✍ P. Bucci; G. Chidichimo; A. Liguori; G. Menniti; N. Uccella 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 300 KB 👁 1 views

Epimeric mixtures of isoHazolidine derivatives were investigated by ' H NMR spectra obtained in the presence of lanthanide shift reagents. The NMR signals of all the components contained in a mixture of 2 , 3 -d i p n e n y l -S -c e ~y ~~~~e s were identified and the LISCA computer program enabled

Measurement of intravascular Na+ during
Measurement of intravascular Na+ during increased CBF using 23Na NMR with a shift reagent
✍ Itamar Ronen; Seong-Gi Kim 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 103 KB

## Abstract Sodium ions are intimately involved with neural activity. Thus, it is highly desirable to devise a way of mapping brain activity via sodium imaging. Sodium ions exist in the extravascular and intravascular spaces. To separate the two components, the shift reagent Tm(DOTP)^5−^ was intrav