Simultaneous Measurement of 3J HN,Hα and 3J Hα,Hβ Coupling Constants in 13 C, 15 N-Labeled Proteins
✍ Scribed by Löhr, Frank; Schmidt, Jürgen M.; Rüterjans, Heinz
- Book ID
- 126924375
- Publisher
- American Chemical Society
- Year
- 1999
- Tongue
- English
- Weight
- 96 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0002-7863
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📜 SIMILAR VOLUMES
Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(HN, H~) and 3J(HN,C') coupling constants in uniformly 13C-and 15N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method
## Abstract We present a pulse sequence for the simultaneous measurement of NH and CαHα couplings in double‐labeled proteins from 2D spectra. The proposed sequence, a modification of the HN(CO)CA experiment, combines the __J__‐modulation method and the IPAP scheme. The couplings can be readily re