An exponential gradient gel with O-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins in a size-dependent fa
Simultaneous Determination of High-Density Lipoprotein, Very Low-Density Lipoprotein and Low-Density Lipoprotein Subclass in Human Serum by Microchip CE
β Scribed by Hua Wang; Chongxu Han; Huimin Wang; Qinghui Jin; Daxin Wang; Li Cao; Guangzhou Wang
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 264 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0009-5893
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Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF). IEF separated VLDL and LDL into two major fractions. The observed optical den
Rinsing of gradient composite acrylamide/agarose gels with 1% Triton X-100 permits the efficient electrotransfer of very-low-density and low-density lipoproteins from the gels and does not appear to interfere with subsequent capture of the lipoproteins by charged nylon membranes. Overall efficiency