Efficient electroblotting of human very-low-density lipoproteins and low-density lipoproteins from composite gradient gels to nylon membranes
✍ Scribed by Lucille T. Lee; Laurence Wong; Paul S. Roheim; James J. Thompson
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 365 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Rinsing of gradient composite acrylamide/agarose gels with 1% Triton X-100 permits the efficient electrotransfer of very-low-density and low-density lipoproteins from the gels and does not appear to interfere with subsequent capture of the lipoproteins by charged nylon membranes. Overall efficiency of the transfer/capture process can approach 95% and does not appear to be significantly affected by total lipoprotein concentrations up to 5000 mg/dl. Direct immunoquantification of transferred apolipoproteins on the membrane is feasible as well. The nylon membranes used, however, must be pretested to ensure capture efficiency.