An exponential gradient gel with O-10% acrylamide and 0.5% agarose was developed for electrophoresis of intact high molecular weight lipoproteins. This system resolves very low density lipoproteins, intermediate density lipoproteins, lipoprotein a, and low density lipoproteins in a size-dependent fa
Determination of kinetic parameters of plasma very-low-density, intermediate-density and low-density lipoproteins
β Scribed by A.H.M. Terpstra
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 452 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0895-7177
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Rinsing of gradient composite acrylamide/agarose gels with 1% Triton X-100 permits the efficient electrotransfer of very-low-density and low-density lipoproteins from the gels and does not appear to interfere with subsequent capture of the lipoproteins by charged nylon membranes. Overall efficiency