Simultaneous Determination of Ascorbic Acid and Dehydroascorbic Acid in Plant Materials by High Performance Liquid Chromatography

✍ Scribed by Michael Tausz; Ilse Kranner; Dieter Grill

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 371 KB
• Volume: 7
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199603)7:2<69::aid-pca290>3.0.co;2-#

✦ Synopsis

The adaptation of an isocratic reversed-phase ion-pairing high performance liquid chromatographic method in combination with derivatization of dehydroascorbic acid by l&phenylenediamine prior to injection for simultaneous determination of ascorbic acid and dehydroascorbic acid in plant materials is presented. Extraction was carried out in meta-phosphoric acid, and hexadecyltrimethylammonium bromide was used as the ion-pairing reagent. Ascorbic acid was detected directly at 248 nm whilst dehydroascorbic acid was simultaneously detected as the l&phenylenediamine derivative at 348 nm. Resolution and accuracy were shown to be satisfactory for naturally occurring amounts in plant materials as demonstrated with extracts of Norway spruce (Picea abies (L.) Karst.) needles.