Determination of nimesulide and hydroxynimesulide in human plasma by high performance liquid chromatography

✍ Scribed by Claudio Glachetti; Anna Tenconi

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 71 KB
• Volume: 12
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199803/04)12:2<50::aid-bmc719>3.0.co;2-b

✦ Synopsis

Two specific methods for the simultaneous determination of nimesulide, a non steroidal anti-inflammatory drug, and its hydroxylated metabolite in human plasma are described. Adopting a high performance liquid chromatographic (HPLC) system with UV detection (230 nm), the compounds, extracted from plasma in acidic medium, were separated on ODS columns under gradient conditions, using a phosphate buffer solution and methanol as mobile phase. For each method column length, gradient rate and composition were appropriately selected. The limit of quantitation was 25 ng/mL for both compounds. The two methods were validated by intraday assays at three concentration levels and applied in kinetic studies in healthy volunteers, during which inter-day assays were carried out confirming their feasibility.