Rapid high-performance liquid chromatographic determination of cefepime in human plasma

✍ Scribed by B. Calahorra; M. A. Campanero; B. Sádaba; J. R. Azanza

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 55 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199906)13:4<272::aid-bmc842>3.0.co;2-0

✦ Synopsis

A simple, rapid and reproducible high-performance liquid chromatographic method for the quantitative determination of cefepime in human plasma was developed. Ceftazidime was used as internal standard. Chromatography was performed on a reversed-phase encapped column (Hypersil BDS C 18 ). The samples, after protein precipitation, were eluted with a mobile phase of acetonitrile-acetate buffer, pH 4 (2.8:97.2, v/v). The detection wavelength was 254 nm. The limit of quantitation of cefepime was 0.5 mg/mL and only 0.5 mL of plasma sample was required for the determination. The average cefepime recoveries over a concentration range of 0.5-500 mg/mL ranged from 98 to 104%. Precision and accuracy did not exceed 5%.