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High-performance liquid chromatographic determination of phenylephrine and tropicamide in human aqueous humor

โœ Scribed by M. J. Galmier; A. M. Frasey; S. Meski; E. Beyssac; J. Petit; J. M. Aiache; C. Lartigue

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
40 KB
Volume
14
Category
Article
ISSN
0269-3879

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โœฆ Synopsis

A high-performance liquid chromatographic method for the simultaneous determination of phenylephrine and tropicamide in human aqueous humor was developed. After centrifugation, an aliquot of the supernatant was injected onto the column and the eluent was monitored at 280 nm then 254 nm after 5 min. Separation was performed on a CN column with 0.01 M Pic B8 (octane sulfonic acid)-acetonitrile (65:35, v/v) as mobile phase. The standard curves were linear in the detection range. The precision of the method (expressed by relative standard deviation) and the accuracy (mean error in per cent) were <5% for both intra- and interassays.

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